Triplex Real-Time PCR without DNA Extraction for the Monitoring of Meningococcal Disease
نویسندگان
چکیده
منابع مشابه
Development of Real-Time PCR Methods for the Detection of Bacterial Meningitis Pathogens without DNA Extraction
Neisseria meningitidis (Nm), Haemophilus influenzae (Hi), and Streptococcus pneumoniae (Sp) are the lead causes of bacterial meningitis. Detection of these pathogens from clinical specimens using traditional real-time PCR (rt-PCR) requires DNA extraction to remove the PCR inhibitors prior to testing, which is time consuming and labor intensive. In this study, five species-specific (Nm-sodC and ...
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ژورنال
عنوان ژورنال: Diagnostics
سال: 2018
ISSN: 2075-4418
DOI: 10.3390/diagnostics8030058